r package Search Results


n a r package seuratwrapper v0 3 5 satija lab  (10X Genomics)
86
10X Genomics n a r package seuratwrapper v0 3 5 satija lab
N A R Package Seuratwrapper V0 3 5 Satija Lab, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pm41576948-314-10-33?v=10X+Genomics
Average 86 stars, based on 1 article reviews
n a r package seuratwrapper v0 3 5 satija lab - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
barnes maze  (Med Associates Inc)
94
Med Associates Inc barnes maze
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
Barnes Maze, supplied by Med Associates Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pm21408206-142-9-11?v=Med+Associates+Inc
Average 94 stars, based on 1 article reviews
barnes maze - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
sodium thiosulfate chem impex  (Chem Impex International)
95
Chem Impex International sodium thiosulfate chem impex
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
Sodium Thiosulfate Chem Impex, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/10__1021_slash_acsapm__1c00101-38-7-9?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
sodium thiosulfate chem impex - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
plexiglas box  (Med Associates Inc)
98
Med Associates Inc plexiglas box
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
Plexiglas Box, supplied by Med Associates Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/10__1523_slash_jneurosci__2184___23__2024-73-18-20?v=Med+Associates+Inc
Average 98 stars, based on 1 article reviews
plexiglas box - by Bioz Stars, 2026-08
98/100 stars
  Buy from Supplier
cell culture  (Thermo Fisher)
91
Thermo Fisher cell culture
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
Cell Culture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pmc09258422__NIHMS1816067___supplement___Supp-57-3-13?v=Thermo+Fisher
Average 91 stars, based on 1 article reviews
cell culture - by Bioz Stars, 2026-08
91/100 stars
  Buy from Supplier
lwdataexplorer r package  (LifeWatch Inc)
90
LifeWatch Inc lwdataexplorer r package
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
Lwdataexplorer R Package, supplied by LifeWatch Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pmc11377692-21-13-9?v=LifeWatch+Inc
Average 90 stars, based on 1 article reviews
lwdataexplorer r package - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
r package glmmml  (RStudio)
90
RStudio r package glmmml
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
R Package Glmmml, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pmc11328520-109-11-14?v=RStudio
Average 90 stars, based on 1 article reviews
r package glmmml - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
ldsep v2.1.4 r package  (RStudio)
90
RStudio ldsep v2.1.4 r package
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
Ldsep V2.1.4 R Package, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pmc11546673-300-8-13?v=RStudio
Average 90 stars, based on 1 article reviews
ldsep v2.1.4 r package - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
r package coseg  (CH Instruments)
90
CH Instruments r package coseg
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
R Package Coseg, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pmc05699967-120-21-13?v=CH+Instruments
Average 90 stars, based on 1 article reviews
r package coseg - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
software r package  (ChangePoint Inc)
90
ChangePoint Inc software r package
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
Software R Package, supplied by ChangePoint Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/10__1051_slash_0004___6361_slash_202245201-316-18-20?v=ChangePoint+Inc
Average 90 stars, based on 1 article reviews
software r package - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
r package 'bioage  (BioAge Labs)
90
BioAge Labs r package 'bioage
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
R Package 'Bioage, supplied by BioAge Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pm39292264-47-1-3?v=BioAge+Labs
Average 90 stars, based on 1 article reviews
r package 'bioage - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
r package 'spdep  (RStudio)
90
RStudio r package 'spdep
Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) <t>Barnes</t> <t>maze</t> analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004
R Package 'Spdep, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+package/pmc07809426__42003_2020_1562_MOESM1_ESM-536-12-21?v=RStudio
Average 90 stars, based on 1 article reviews
r package 'spdep - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) Barnes maze analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004

Journal: PLoS genetics

Article Title: FUS transgenic rats develop the phenotypes of amyotrophic lateral sclerosis and frontotemporal lobar degeneration.

doi: 10.1371/journal.pgen.1002011

Figure Lengend Snippet: Figure 4. Neuron loss accompanied by ubiquitin aggregation in aged rats overexpressing the normal human fus gene. (A–C) Double- fluorescence staining for human FUS (A, red) and the neuronal marker NeuN (B, green) in the frontal cortex of normal human FUS transgenic rats (line 20). Most FUS-positive cells expressed NeuN, but some did not (C). (D–I) Immunofluorescence staining revealed ubiquitin aggregation in aged (G–I; 1 year of age), but not young (D–F; 3 months of age), normal FUS transgenic rats. Coronal sections of frontal cortex were immunostained with antibodies to ubiquitin (D, G: green) and human FUS (E, H: red). Scale bars: A–C, 100 mm; D–I, 20 mm. (J, K) Barnes maze analysis revealed spatial learning deficits in normal FUS transgenic rats of line 20 at advanced ages. One year old transgenic rats (TG) and their nontransgenic littermates (NT) were tested in a Barnes maze and time spent to locate the fixed escape hole (latency) and the number of errors made before escaping were recorded. (L, M) Stereological cell counting revealed a loss of neurons in the cortex (J) and dentate gyrus (K) of normal FUS transgenic rats at advanced ages (line 20). Coronal sections of one hemisphere were stained with Cresyl violet and the number of neurons in the frontal cortex and dentate gyrus was estimated by stereological cell counting. Normal FUS transgenic rats and their nontransgenic controls were killed at the age of 1 year. Data are presented as means 6 SD (n = 5). * p,0.05. doi:10.1371/journal.pgen.1002011.g004

Article Snippet: Spatial learning and memory tasks were examined with a Barnes Maze (Med Associates).

Techniques: Ubiquitin Proteomics, Fluorescence, Staining, Marker, Transgenic Assay, Immunofluorescence, Cell Counting